AST, Ast, or ast may refer to:
Science and technology
- Attention schema theory, of consciousness or subjective awareness
 
Computing
- Abstract syntax tree, a finite, labeled, directed tree used in computer science
 - Anamorphic stretch transform, a physics-inspired signal transform
 - Andrew S. Tanenbaum (born 1944), American-Dutch computer scientist, sometimes identified as "ast"
 - Asynchronous System Trap, a mechanism used in several computer operating systems
 - Application Security Testing, testing the operation and security of a software application
 - Application Support Team, a team of Application Support Analysts supporting IT services delivered to users within an organisation, enabling the required operational processes needed for the business to be successful.
 
Medicine
- Aspartate transaminase, an enzyme associated with liver parenchymal cells
 - Antibiotic sensitivity testing or antibiotic susceptibility testing, the measurement of the susceptibility of bacteria to antibiotics
 
Mathematics
Organizations
- Allied Security Trust, a patent holding company
 - AST Research, a defunct personal computer manufacturer
 - Association for Software Testing, US
 - AST (publisher), a Russian book publishing company
 - Arsenal Supporters' Trust, English football club supporters
 
Politics and government
- Abwehrstelle or Ast, the local intelligence center in each military district in Nazi Germany
 - Alaska State Troopers, state police agency in Alaska, US
 - Chadian Social Action (French: Action Sociale Tchadienne), a former political party in Chad
 - Office of Commercial Space Transportation, of the US FAA
 
Education
- ACT Scaling Test, for year 12 students, Australia
 - Advanced Skills Teacher, a teaching role in England and Wales
 - Air Service Training, a flight engineering training school
 - American School in Taichung, an international school in Taichung, Taiwan
 - American School of Tangier
 - American School of Tegucigalpa
 - American School of Tripoli
 - Atlantic School of Theology, Halifax, Nova Scotia, Canada
 - Avalanche Skills Training, Canada
 - Conroe ISD Academy of Science and Technology, Texas, US
 
Transport
- Aldershot GO Station, Amtrak station code AST
 - Astoria Regional Airport (FAA Identifier and IATA code 
AST), Clatsop County, Oregon, US - Aston railway station (National Rail code), Birmingham, England
 
Time zones
- Atlantic Standard Time, UTC−4
 - Alaska Standard Time, UTC−9
 - Arabia Standard Time, UTC+3
 - Antigua and Barbuda Time, UTC−4
 
Other uses
- Aviation Survival Technician, a US Coast Guard rating
 - AST Research, Inc., as known as AST Computer, a personal computer manufacturer
 - Asti, Italian province and city (was traditionally Ast in the Piemontese dialect)
 - Assured shorthold tenancy, UK
 - Asturian language (ISO 639 alpha-3: 
ast), Spain - Astana Pro Team (UCI code AST), a professional road bicycle racing team
 - Georg Anton Friedrich Ast, German philosopher and philologist (1778–1841)
 - Pat Ast, American actress and model (1941-2001)
 
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