DHF may refer to:
- Al Dhafra Air Base (IATA code DHF)
- Danish Handball Federation (Dansk Håndbold Forbund), the national governing body for handball in Denmark
- Design history file, documentation of the design history of a medical device, kept per FDA regulations
- Dihydrofolic acid, a folic acid derivative that occurs in some metabolic pathways
- Diastolic heart failure, heart failure during diastole
- Decompensated heart failure, that is, acute decompensated heart failure, a sudden worsening of heart failure
- Dengue hemorrhagic fever, the severe form of dengue fever with hemorrhage and high mortality risk
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