Tsetse and similar can mean :
- Tsetse fly, one of the large biting flies inhabiting much of mid-continental Africa between the Sahara and the Kalahari deserts
- Tse Tse Fly (band), a late 1980s/early 1990s British rock band
- De Havilland Mosquito, FB Mk XVIII version was nicknamed Tsetse
- Tsetse (nuclear primary), the common design nuclear fission bomb core for several Cold War designs for American nuclear and thermonuclear weapons
- Tsetse, North West, a village in South Africa
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